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شركــــــة Bio Rad   الامريكــــــــة 

متخصصة في انتاج الاجهزة المختبرية و التحليلية  مثل

 Real Time PCR, Gradient PCR, GEL Documentary, Centrifuges, Elisa Systems and others

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Bio Rad / USA

Specialized in manufacturing Life Science products such as Real Time PCR, Gradient PCR, GEL Documentary, Centrifuges, Elisa Systems and others.

Website: www.bio-rad.com

PCR systems: First generation: Conventional PCR

Second generation: Real time PCR

Real Time PCR CFX96

The CFX96™ optical reaction module converts the C1000 Touch™ thermal cycler chassis into the powerful and precise CFX96 Touch real-time PCR detection system. This six-channel system combines advanced optical technology with precise thermal control to deliver sensitive, reliable detection. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to five-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX96 Touch system is designed to advance your qPCR.

Key Features and Benefits

With the CFX96 Touch real-time PCR detection system you can:

Set up your system quickly — easy installation and factory-calibrated optics

Minimize sample and reagent use — up to 5-target multiplexing with sample volumes as low as 10 µl

Optimize reactions in a single run — thermal gradient feature

Analyze data faster — visualize all run data at once and export only the data you need in the format you want

Use advanced data analysis tools — normalized gene expression using CFX Manager™ software

Configure the system to fit your needs — run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX automation system for higher throughput

Streamline publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbasePLUS software

The CFX automation system works with the CFX Connect, CFX96 Touch, and CFX384 Touch real-time PCR detection systems to enable walk-away, high-throughput operation in a simplified format that does not compromise precision. The plug-and-go benchtop plate handler has the capacity to load up to twenty 384-well PCR plates at a time.

This system facilitates the automation of workflows, generation of large volumes of data, and rapid data analysis. It is ideally suited to meet the high-throughput requirements of today’s drug discovery workflows, letting you process up to 7,680 samples in a single run.

The CFX automation system makes it easy to:

·         Maximize laboratory throughput by integrating one CFX96 or CFX384 real-time PCR detection system with hands-free running of up to 20 plates

·         Improve experimental workflow and automation tasks to ensure maximum productivity

·         Track samples using the integrated bar code reader

·         Navigate, set up, and execute multiple PCR experiments using intuitive CFX automation control software

·         Define PCR protocols for an entire plate stack at once

·         Receive email notification with an attached data file or report upon completion of a single run

C1000 Touch thermal cycler

The C1000 Touch thermal cycler offers superior performance and a large color touch screen for easy programming. This fully modular platform is able to accommodate different throughput needs with easily interchangeable reaction modules that swap in seconds without tools. Each PCR module has a fully adjustable heated lid that supports a wide range of vessels and sealers, including low-profile and standard-height PCR plates.

Features and Benefits

Quick and easy protocol programming — 2 programming options, including the Protocol Autowriter, which automatically generates customized standard, fast, and ultrafast protocols

Easy protocol editing — the large color touch screen and redesigned navigation make editing and running protocols easy

Flexible platform — change throughput capability by linking up to 3 additional S1000™ thermal cyclers or adding PC control for up to 32 cyclers

Advanced file management — USB flash drive compatibility allows universal protocol transfer and unlimited data storage

Thermal gradient — quickly and easily identify optimal annealing temperatures by using the programmable temperature gradient.

The T100 thermal cycler is a small thermal cycler offering a comprehensive set of convenient features in a small footprint. This compact thermal cycler features an intuitive touch-screen user interface to make running PCR easy. Thermal gradient technology allows you to quickly optimize your reaction in a single run. With its robust design, the T100 system is a reliable personal thermal cycler that delivers exceptional performance for years — test drive the T100 thermal cycler today.

Features and Benefits of the T100 Thermal Cycler

Intuitive touch screen — easy programming using a large color touch screen and intuitive interface

Consistent results — robust design ensures effective and consistent performance run to run

Easy optimization — this gradient thermal cycler allows fast PCR optimization using a unique thermal gradient

Easy protocol management — protocols can be organized using personalized folders or a USB flash drive

Small space-saving footprint — the T100 is a compact thermal cycler that fits in any laboratory

Third generation: Droplet digital PCR

QX-100 Droplet digital PCR

Bio-Rad Laboratories presents Droplet Digital PCR (ddPCR) a novel combination of limiting dilutions, end-point PCR, and Poisson statistics, yielding absolute measurements of nucleic acid concentration. By generating uniformed mono-dispersed droplets and using conventional probe based assays within a 96- well plate environment, our system provides data orders of magnitude more precise and sensitive than traditional qPCR. Bio-Rad's instrument, the QX100, exceeds the ability of traditional qPCR techniques.A Breakthrough in Quantitative PCR:

Droplet Digital™ PCR

• Rare Event Detection

Mutation detection down to 0.001%

Single-cell PCR detection

• Copy Number Variations

Structural genomics

Aneuploidy/Translocations

• Genotyping

• Absolute Quantification

No standard curve needed, no relative quantification

Single copy resolution

Unambiguous genotype calling

Applications and Uses

·         Copy number variation

·         Rare sequence detection

·         Mutation detection

·         Gene expression analysis

·         miRNA analysis

·         Next generation sequencing sample quantification

Specifications for the QX100™ Droplet Digital™ PCR system

The PX1 PCR plate sealer provides consistent and uniform sealing across an entire microplate. This semiautomated heat sealer is ready to seal in less than 3 minutes. The PX1 sealer removes human variability from plate sealing and minimizes sample evaporation from the PCR workflow. This versatile instrument is compatible with a variety of heat sealing films and foils and a wide range of PCR plates.

The PX1 PCR plate sealer features an intuitive touch-screen user interface. The thermal sealing process is simplified by allowing sealing temperature and time to be modified with the touch of a button.

Key Features and Benefits

·         Fast startup time — avoid delaying an experiment while the sealer warms up

·         Extremely intuitive — save time programming the instrument

·         Small footprint — accommodates crowded laboratory benches

·         Quickly access sealing protocols — save time by easily using stored protocols

NGC family of chromatography system

The family of NGC medium-pressure chromatography systems offers a single laboratory chromatography solution that scales to fit your throughput requirements. Preconfigured systems are available and are easily upgradable as your throughput and automation needs change.

The NGC system’s modular design provides multiple configurations that each align with the requirements of individual scientists. The system’s small footprint design allows the instrument to be used on a lab bench, in a laboratory refrigerator, or in a coldroom.

The preconfigured NGC Quest™ 10, NGC™ Quest 10 Plus, NGC Scout™ 10, NGC™ Scout 10 Plus, and NGC Discover™ 10 systems are designed with increasing automation and throughput to serve a wide range of laboratory needs. Additionally, each system is also completely open to individual customization through the selection of applicable modules suitable to the application at hand. To learn more, watch the NGC system tour.

Prepacked Analytical-Scale Chromatography Media Cartridges by Application

Common Chromatography Method Elements

Flow cytometer: S3 Cell sorter

The S3 cell sorter is the first truly walk-up automated cell sorter available to scientists. Expertly engineered with revolutionary precision and automation, the S3 cell sorter is a benchtop cell sorter equipped with one or two lasers and up to four fluorescent detectors plus scatter detectors utilizing traditional jet-in-air technology. Samples can be sorted at high speeds while maintaining sensitivity and high purity. Cell sorting has never been easier for the most common sorting experiments. Small in footprint, the S3 cell sorter brings affordable, dependable, and simplified cell sorting to every researcher.

Key Features and Benefits

Walk-up cell sorting — provides automation to simplify setup and optimization

Automated setup — creates precision sorting without user input or constant monitoring; fully automated drop delay calculation, stream-to-laser alignment, droplet break-off monitoring, and feedback ensure sort accuracy

Benchtop footprint — includes space-saving, internalized fluidics and temperature control system to fit in any lab

High speed and high purity — sorts cells faster while maintaining high sensitivity and yield

2-way cell sorting — sorts 2 different defined populations at the same time

Automatic cleaning — reduces carryover with a dual-position loading stage that ensures the sample line is clean between sort runs

Specifications For Flow cytometer: S3 Cell sorter

With its unique imaging capabilities, monochromator design, and spectral scanning feature (which eliminates the need for filters), the xMark™ microplate absorbance spectrophotometer can find the best wavelength within a broad range for any photometric application. The xMark spectrophotometer is also flexible enough to read all standard microplates, from 6- to 1,536-well formats. Using Microplate Manager^® software, the xMark spectrophotometer can perform a wide range of end-point and kinetic applications with spectral scanning within low UV to infrared wavelengths. Extensive data analysis includes comprehensive curve-fit models and automated data export in single-plate or batch formats. Benefits include:

·         Monochromator-based system for maximum optical efficiency and flexibility in photometry reading

·         Spectral scanning to define the best absorbance wavelength for assays

·         Imaging capabilities for pixel intensity data in a single well or entire plate

·         Ability to scan custom plates

·         Flexible control and data analysis with PC or Mac software

·         Incubator with programmable temperature control

The ImmunoWash™ 1575 microplate washer is essential for performing accurate and reproducible immunoassays in a microplate format, such as ELISA.

Features and Benefits

·         Choose from a variety of preprogrammed wash sequences

·         Design, store, and reproducibly perform special wash routines

·         Maximize wash efficiency by adapting to various liquids and buffers, assay components and conditions

·         Exercise complete control of needle position (horizontal and vertical) and aspiration/dispensing rates

Applications and Uses

·         Suitable for autoclave sterilization

·         Compatible with 96-well microplates that have flat-, U-, or V-bottom wells and strips

·         Registered with the FDA as a medical device for various clinical chemistry applications

Specifications for Micro plate Absorbance Spectrophotometer (Elisa Systems)

Immunowash Microplate Washer

 ChemiDoc™ XRS+ System & ChemiDoc MP System

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection

methods. The system is controlled by Image Lab™ software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.

Features and Benefits

Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection

Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting

High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation

Superior Image Quality — Exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds.

Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height; automated hands-free operation ensures consistent, reproducible, and high-throughput performance

 Specifications

Gel Doc EZ System

The Gel Doc EZ system is a compact and automated gel imaging instrument designed to yield publication-quality images and analyzed results with just the push of a button. The Gel Doc EZ system provides unparalleled flexibility with the use of four application-specific trays:

UV tray for ethidium bromide staining of DNA gels and fluorescence imaging

White tray for Coomassie, copper, silver, and zinc stains

Blue tray for nucleic acid applications with SYBR^® stains

Stain-free tray for stain-free imaging with Bio-Rad stain-free gels

The intuitive Image Lab™ software bundled with the Gel Doc EZ gel imaging system includes capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy.

Features and Benefits of the Gel Doc EZ system

Easy to use — Researchers unfamiliar with the Gel Doc EZ imaging system can learn to use it quickly and easily because there is no need for manual control of filters, lens, or lights. Create a default protocol once, then use the green button on the front of the instrument to use these gel imaging and analysis settings time and again.

Publication-quality images and full data analysis — Get clean and smooth publication-ready images with decreased pixelation when images are cropped or zoomed. Output analyzed results including relative molecular weights, bands quantitation, Excel reports, PDFs, and more in a matter of minutes.

Time and space savings — Obtain results faster without spending time on various steps in image acquisition and save valuable bench space using this compact system.

Modular design and flexible options — Use specific trays for different gel imaging applications. Clearly defined and color-coded trays eliminate confusion. Purchase only what you need and upgrade as your research needs change.

Stain-free technology — Condense your 2 hr Coomassie protocol into a 5 min stain-and-image step with this stain-free gel imaging system. Bio-Rad stain-free gels are western blot compatible, allowing you to check electrophoresis results and quality prior to western blotting. These “green” gels provide equal or better sensitivity than Coomassie staining and eliminate organic waste disposal problems.

Electrophoresis:

Experion Automated Electrophoresis System

The Experion automated electrophoresis station performs all of the steps of gel-based electrophoresis in one compact, durable unit. It automates analysis by combining electrophoresis, staining, destaining, band detection, and imaging into a single, 30 min step. It's an electrophoresis cell, power supply, and imager built into a single device and is designed for easy, precise operation:

Highly accurate laser provides precise fluorescence detection

USB port allows easy installation and maximum usability

Built-in power supply reduces cost and saves bench space

The following types of kits are available for analysis of proteins, RNA, and DNA:

Experion starter kits

Experion protein analysis kits

Experion RNA analysis kits

Experion DNA analysis kits

The Experion automated electrophoresis system can be used upstream and downstream of a number of other nucleic acid- and protein-application focused products from Bio-Rad.

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